Genetic Analysis of the Mitochondrial rrnS Gene of Zoonotic Anisakis pegreffii (Nematoda: Anisakidae) Isolated from Micromesistius Poutassou (R.) in the Aegean Sea

Ascaridoid nematodes were found in viscera of the blue whiting Micromesistius poutassou (Risso, 1826) from Aegean Sea parasitological examination. Collected morphologically identified as third (L3) stage Anisakis type I larvae and then subject to Restriction Fragment Length Polymorphism (RFLP) analysis internal transcribed spacer (ITS) region identify species. Randomly selected molecularly confirmed pegreffii by RFLP analysis. Subsequently, L3 A. amplified bi-directionally sequenced at small subunit ribosomal RNA (rrnS) gene. The rrnS sequence (MT484284) had 100% identity with gene (JX500050, LC222461, MF140359, MT312511, MT312512) which fish cetaceans hosts, Scomber japonicus, Conger myriaster, Neophocaena asiaeorientalis, Stenella coeruleoalba, Mediterranean Sea, Japanese, Chinese, Korean waters. This study provided genetic zoonotic Turkish marine waters based on for first time. can be used novel ascaridoid Sea.